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JAM-A / F11R Anticorpo, Anticorpo monoclonal de Ratazanao

Folha de dadosAnálisesProdutos relacionadosProtocolos
Human F11R Informação sobre produtos de anticorpos
Imunogénio:Recombinant Human JAM-A / F11R / CD321 protein (Catalog#10198-H08H)
Clone ID:04
Tipo Ig:Mouse IgG1
Concentração:
Endotoxina:
Formulação:0.2 μm filtered solution in PBS with 5% trehalose
Preparação:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAM-A / F11R / CD321 (rh JAM-A / F11R / CD321; Catalog#10198-H08H; NP_058642.1; Met1-Ala242). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human F11R Guia de utilização de anticorpos
Especificidade:Human JAM-A / F11R / CD321
Aplicação:ICC/IF, IF, FCM

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

Armazenamento:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human F11R Antibody IHC Application Image
JAM-A / F11R Antibody, Mouse MAb, Immunofluorescence
[Clicar para ampliar a imagem]
Caption:
Immunofluorescence staining of Human JAM-A in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human JAM-A monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human F11R Antibody FC Application Image
Junctional Adhesion Molecule A Antibody, Mouse MAb, Flow cytometric analysis
[Clicar para ampliar a imagem]
Caption:
Flow cytometric analysis of human JAM-A(CD321) expression on SKBR3 cells. Cells were stained with purified anti-Human JAM-A(CD321), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Other F11R Antibody Products
Junctional Adhesion Molecule A Fundo

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

Human Junctional Adhesion Molecule A Referências
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
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