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Gripe B (B/Malaysia/2506/2004) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His Etiqueta

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Influenza B HA Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:
Tamanho de cDNA:1758bp
Descrição de cDNA:Full length Clone DNA of Influenza B (B/Malaysia/2506/2004) HA with N terminal His tag.
Sinónimo de gene:HA1, Hemagglutinin
Espécie:Influenza B
Vetor:pCMV3-SP-N-His
Plasmid:pCMV3-His-B-MA-04-HA
Local de restrição:KpnI + XbaI (6kb + 1.80kb)
Sequência de etiqueta:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Descrição da sequência:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ACO05957.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at ambient temperature for three months.
Influenza B HA Gene Plasmid Map
Influenza B (B/Malaysia/2506/2004) Hemagglutinin (Codon Optimized) natural ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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  • Influenza B (B/Malaysia/2506/2004) Hemagglutinin (Codon Optimized) natural ORF mammalian expression plasmid, N-His tag
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