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Humano UGT2A3 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta

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    Humano UGT2A3 Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:NM_024743.3
    Tamanho de cDNA:1584bp
    Descrição de cDNA:Full length Clone DNA of Homo sapiens UDP glucuronosyltransferase 2 family, polypeptide A3 with N terminal Myc tag.
    Sinónimo de gene:UGT2A3
    Espécie:Human
    Vetor:pCMV3-SP-N-Myc
    Plasmid:
    Local de restrição:
    Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    Descrição da sequência:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with UGT2A3 qPCR primers for gene expression analysis, HP102418 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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