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Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta

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Human SUMO1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_003352.4
Tamanho de cDNA:306bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens small ubiquitin-like modifier 1 with N terminal Myc tag.
Sinónimo de gene:DAP1, GMP1, PIC1, SMT3, UBL1, OFC10, SENP2, SMT3C, SMT3H3
Espécie:Human
Vetor:pCMV3-N-Myc
Plasmid:
Local de restrição:
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta on other vectors
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG13095-ACG$225
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG13095-ACR$225
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaHG13095-ANG$225
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaHG13095-ANR$225
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG13095-CF$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG13095-CH$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG13095-CM$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG13095-CY$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de expressão)HG13095-G$75
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG13095-NF$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG13095-NH$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG13095-NM$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG13095-NY$195
Humano SUMO1/SUMO-1 clonagem de ADN ou de clonagem do gene (vector de clonagem)HG13095-UT$195
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Fundo

Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.

Referências
  • Kim HJ, et al. (2011) SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2. Biochem Biophys Res Commun. 410(3): 555-62.
  • Zuo Y, et al. (2009) Small ubiquitin-like modifier protein-specific protease 1 and prostate cancer. Asian J Androl. 11(1): 36-8.
  • Song T, et al. (2008) SUMO1 polymorphisms are associated with non-syndromic cleft lip with or without cleft palate. Biochem Biophys Res Commun. 377(4): 1265-8.
  • Katayama A, et al. (2007) Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: possible involvement in tumor proliferation and prognosis. Int J Oncol. 31(3): 517-24.
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