After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Encomenda rápida

Text Size:AAA

Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta

Folha de dadosAnálisesProdutos relacionadosProtocolos
Human SDC1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_001006946.1
Tamanho de cDNA:933bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens syndecan 11 with N terminal Myc tag.
Sinónimo de gene:SDC, CD138, SYND1, syndecan, SDC1
Espécie:Human
Vetor:pCMV3-SP-N-Myc
Plasmid:pCMV3-Myc-SDC1
Local de restrição:KpnI + XbaI (6kb + 0.96kb)
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 279G/A not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Human SDC1 Gene Plasmid Map
Human SDC1 ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta on other vectors
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG11429-ACG$225
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG11429-ACR$225
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG11429-CF$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG11429-CH$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG11429-CM$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG11429-CY$195
Humano SDC1 Gene cDNA clone plasmidHG11429-M$75
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG11429-M-H$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG11429-NF$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG11429-NH$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG11429-NM$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG11429-NY$195
Humano Syndecan-1/CD138/SDC1 clonagem de ADN ou de clonagem do gene (vector de clonagem)HG11429-UT$195
 Saiba mais sobre vectores de expressão
Product nameProduct name
Fundo

Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.

Referências
  • Inki P, et al. (1996) The role of syndecan-1 in malignancies. Ann Med. 28(1): 63-7.
  • Subramanian SV, et al. (1997) Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation. J Biol Chem. 272(23): 14713-20.
  • Park PW, et al. (2001) Exploitation of syndecan-1 shedding by Pseudomonas aeruginosa enhances virulence. Nature. 411(6833): 98-102.
  • Size / Price
    Catálogo: HG11429-NM
    Preço de catálogo: 
    Preço:      (You Save: )
    Tamanho:
    Quantidade:+-
    DisponibilidadeIn Stock
    Bulk Discount RequiryAcrescentar a carrinho
    Contact Us
    Imagens
    • Human SDC1 ORF mammalian expression plasmid, N-Myc tag
      Itens recentemente visualizados
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Tenha em atenção o seguinte: Todos os produtos destinam-se "A FINS EXCLUSIVOS DE INVESTIGAÇÃO E NÃO DEVEM SER UTILIZADOS PARA EFEITOS DE DIAGNÓSTICO OU TERAPÊUTICOS"