|Folha de dados||Análises||Produtos relacionados||Protocolos|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PDZD11 ORF mammalian expression plasmid, C-GFPSpark tag||HG12188-ACG|
|Human PDZD11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12188-ACR|
|Human PDZD11 ORF mammalian expression plasmid, C-Flag tag||HG12188-CF|
|Human PDZD11 ORF mammalian expression plasmid, C-His tag||HG12188-CH|
|Human PDZD11 ORF mammalian expression plasmid, C-Myc tag||HG12188-CM|
|Human PDZD11 ORF mammalian expression plasmid, C-HA tag||HG12188-CY|
|Human PDZD11 Gene cDNA clone plasmid||HG12188-G|
|Human PDZD11 ORF mammalian expression plasmid, N-Flag tag||HG12188-NF|
|Human PDZD11 ORF mammalian expression plasmid, N-His tag||HG12188-NH|
|Human PDZD11 ORF mammalian expression plasmid, N-Myc tag||HG12188-NM|
|Human PDZD11 ORF mammalian expression plasmid, N-HA tag||HG12188-NY|
|Human PDZD11 natural ORF mammalian expression plasmid||HG12188-UT|
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PDZ domain-containing protein 11, also known as AIPP1a, PISP, PDZD11 and PDZK11, is a cytosolic protein which contains one PDZ (DHR) domain. PDZD11 bears resemblance to members of the MALS / VELIS family of proteins. It contains but one PDZ domain that apparently interacts with the C-terminus of partner proteins. PDZD11 is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. PDZD11 is a transiently interacting partner of the PMCA b-splice forms that may play a role in their sorting to or from the plasma membrane. Full-length human PDZD11 shares 97% amino acids (aa) identity with mouse PDZD11.