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Humano LIPG clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta

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Humano LIPG Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:BC060825
Tamanho de cDNA:1503bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens lipase, endothelial with N terminal Myc tag.
Sinónimo de gene:EL, EDL, PRO719, LIPG
Espécie:Human
Vetor:pCMV3-SP-N-Myc
Plasmid:pCMV3-Myc-LIPG
Local de restrição:KpnI + XbaI (6kb + 1.54kb)
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with LIPG qPCR primers for gene expression analysis, HP102411 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Humano LIPG Gene Plasmid Map
Human LIPG ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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  • Human LIPG ORF mammalian expression plasmid, N-Myc tag
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Tenha em atenção o seguinte: Todos os produtos destinam-se "A FINS EXCLUSIVOS DE INVESTIGAÇÃO E NÃO DEVEM SER UTILIZADOS PARA EFEITOS DE DIAGNÓSTICO OU TERAPÊUTICOS"