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Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His Etiqueta

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Human LGMN Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:BC003061
Tamanho de cDNA:1302bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens legumain with N terminal His tag.
Sinónimo de gene:AEP, LGMN1, PRSC1
Espécie:Human
Vetor:pCMV3-N-His
Plasmid:
Local de restrição:
Sequência de etiqueta:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His Etiqueta on other vectors
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG12846-ACG$225
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG12846-ACR$225
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaHG12846-ANG$225
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaHG12846-ANR$225
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG12846-CF$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG12846-CH$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG12846-CM$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG12846-CY$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de expressão)HG12846-G$75
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG12846-NF$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG12846-NH$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG12846-NM$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG12846-NY$195
Humano Legumain clonagem de ADN ou de clonagem do gene (vector de clonagem)HG12846-UT$195
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Fundo

The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.

Referências
  • Schwarz G, et al. (2002) Characterization of legumain. Biol Chem. 383(11): 1813-6.
  • Liu C, et al. (2003) Overexpression of legumain in tumors is significant for invasion/metastasis and a candidate enzymatic target for prodrug therapy. Cancer Res. 63(11): 2957-64.
  • Murthy RV, et al. (2005) Legumain expression in relation to clinicopathologic and biological variables in colorectal cancer. Clin Cancer Res. 11(6): 2293-9.
  • Gawenda J, et al. (2007) Legumain expression as a prognostic factor in breast cancer patients. Breast Cancer Res Treat. 102(1): 1-6.
  • Clerin V, et al. (2007) Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis. 201(1): 53-66.
  • Lew?“n S, et al. (2008) A Legumain-based minigene vaccine targets the tumor stroma and suppresses breast cancer growth and angiogenesis. Cancer Immunol Immunother. 57(4): 507-15.
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    Catálogo: HG12846-NH
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    Disponibilidade2-3 weeks
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