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Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta

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    Humano GZMB Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:BC030195
    Tamanho de cDNA:744bp
    Descrição de cDNA:Full length Clone DNA of Homo sapiens granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) with N terminal Flag tag.
    Sinónimo de gene:HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZMB
    Espécie:Human
    Vetor:pCMV3-N-FLAG
    Plasmid:
    Local de restrição:
    Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descrição da sequência:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with GZMB qPCR primers for gene expression analysis, HP100383 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta on other vectors
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG13352-ACG$225
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    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaHG13352-ANG$225
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaHG13352-ANR$225
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG13352-CF$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG13352-CH$195
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    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG13352-CY$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de expressão)HG13352-G$75
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG13352-G-H$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG13352-NF$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG13352-NH$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG13352-NM$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG13352-NY$195
    Humano Granzyme B/GZMB clonagem de ADN ou de clonagem do gene (vector de clonagem)HG13352-UT$195
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    Fundo

    Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

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