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Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta

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Human ENSA Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:BC000436
Tamanho de cDNA:366bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens endosulfine alpha with N terminal Myc tag.
Sinónimo de gene:RP11-54A4.10-004, ARPP-19e, MGC4319, MGC78563, MGC8394, ENSA
Espécie:Human
Vetor:pCMV3-N-Myc
Plasmid:
Local de restrição:
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc Etiqueta on other vectors
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG14321-ACG$225
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG14321-ACR$225
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaHG14321-ANG$225
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaHG14321-ANR$225
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG14321-CF$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG14321-CH$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG14321-CM$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG14321-CY$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de expressão)HG14321-G$75
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG14321-NF$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG14321-NH$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG14321-NM$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG14321-NY$195
Humano ENSA / Endosulfine alpha clonagem de ADN ou de clonagem do gene (vector de clonagem)HG14321-UT$195
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Fundo

Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Referências
  • Ye M. et al., 2001, Genome Res. 10 (10): 1546-60.
  • Apiou F. et al., 1999, Diabetes 48 (9): 1873-6.
  • Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.
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    Catálogo: HG14321-NM
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    Disponibilidade2-3 weeks
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