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Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His Etiqueta

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Human CSK Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_001127190.1
Tamanho de cDNA:1353bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens c-src tyrosine kinase with N terminal His tag.
Sinónimo de gene:MGC117393, CSK
Espécie:Human
Vetor:pCMV3-N-His
Plasmid:
Local de restrição:
Sequência de etiqueta:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His Etiqueta on other vectors
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG10740-ACG$225
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG10740-ACR$225
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaHG10740-ANG$225
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaHG10740-ANR$225
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG10740-CF$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG10740-CH$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG10740-CM$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG10740-CY$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de expressão)HG10740-M$75
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG10740-NF$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG10740-NH$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG10740-NM$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG10740-NY$195
Humano C-Src Kinase / CSK clonagem de ADN ou de clonagem do gene (vector de clonagem)HG10740-UT$195
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Fundo

The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.

References

Referências
  • Brauninger A. et al.,1992, Gene. 110: 205-11.
  • Sondhi D. et al., 1999, Biochemistry. 38 (34): 11147-55.
  • Ogawa A. et al., 2002, J Biol Chem. 277 (17): 14351-4.
  • Cole PA. et al., 2003, Curr Opin Chem Biol. 7 (5): 580-5.
  • Baumeister U. et al., 2005,EMBO J. 24 (9): 1686-95.
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