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Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta

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    Humano CIRBP Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:BC000403
    Tamanho de cDNA:519bp
    Descrição de cDNA:Full length Clone DNA of Homo sapiens cold inducible RNA binding protein with N terminal Flag tag.
    Sinónimo de gene:CIRP
    Espécie:Human
    Vetor:pCMV3-N-FLAG
    Plasmid:
    Local de restrição:
    Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descrição da sequência:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with CIRBP qPCR primers for gene expression analysis, HP103214 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta on other vectors
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG14578-ACG$225
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG14578-ACR$225
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaHG14578-ANG$225
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaHG14578-ANR$225
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG14578-CF$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG14578-CH$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG14578-CM$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG14578-CY$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de expressão)HG14578-G$75
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG14578-NF$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG14578-NH$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG14578-NM$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG14578-NY$195
    Humano CIRBP / Cold-inducible RNA-binding Proteína clonagem de ADN ou de clonagem do gene (vector de clonagem)HG14578-UT$195
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    Fundo

    CIRBP, also known as cold-inducible RNA-binding protein, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP responds to a wide array of cellular stresses, including short wavelength ultraviolet light (UVC), at the transcriptional and post-translational level. It acts as a translational activator.CIRBP can bind the 3 translated region of specific transcripts to stabilize them and facilitate their transport to ribosomes for translation. CIRBP affects NF-κB signaling as opposed to IL1B mRNA stability directly.

    Referências
  • Artero-Castro A. et al., 2009, Mol Cell Biol. 29 (7): 1855-68.
  • Zeng Y. et al., 2009, J Cell Biochem. 107 (1): 179-88.
  • Brochu C. et al., 2013, PLoS One. 8 (2): e57426.
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    Catálogo: HG14578-NF
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