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Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His Etiqueta

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Human CES3 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_024922.4
Tamanho de cDNA:1716bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens carboxylesterase 3 with C terminal His tag.
Sinónimo de gene:ES31, FLJ21736, CES3
Espécie:Human
Vetor:pCMV3-C-His
Plasmid:
Local de restrição:
Sequência de etiqueta:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His Etiqueta on other vectors
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG11129-ACG$245
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG11129-ACR$245
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG11129-CF$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG11129-CH$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG11129-CM$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG11129-CY$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de expressão)HG11129-M$75
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG11129-M-F$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG11129-NF$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG11129-NH$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG11129-NM$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG11129-NY$215
Humano CES3/Carboxylesterase 3 clonagem de ADN ou de clonagem do gene (vector de clonagem)HG11129-UT$215
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Fundo

Carboxylesterases hydrolyze esters of short-chain fatty acids and have roles in animals ranging from signal transduction to xenobiotic detoxification. In enzymology, a carboxylesterase is an enzyme that catalyzes the chemical reaction: a carboxylic ester + H2O = an alcohol + a carboxylate. Most enzymes from this group belong to the superfamily of hydrolases with alpha/beta protein fold (so called Alpha/beta hydrolase fold), specifically those acting on carboxylic ester bonds. The carboxylesterase family of evolutionarily related proteins (those with clear sequence homology to each other) includes a number of proteins with different substrate specificities, such as acetylcholinesterases. Carboxylesterase 3, also known as Liver carboxylesterase 31 homolog and CES3, is a endoplasmic reticulum lumen which belongs to the type-B carboxylesterase/lipase family. CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES3 shows low catalytic efficiency for hydrolysis of CPT-11, a prodrug for camptothecin used in cancer therapeutics. CES3 is expressed in liver, colon and small intestine.

Referências
  • Augusteyn RC. et al.,1969, Biochim Biophys Acta. 171 (1): 128-37.
  • Saito S. et al., 2003, J. Hum. Genet. 48: 249-70.
  • Sanghani SP. et al., 2003, Clin Cancer Res. 9: 4983-91.
  • Sanghani SP. et al., 2004, Drug Metab Dispos. 32: 505-11.
  • Chen R. et al., 2009, J Proteome Res. 8: 651-61.
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    Catálogo: HG11129-CH
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