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Humano BUB3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc Etiqueta

  • Human BUB3 ORF mammalian expression plasmid, C-Myc tag
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Humano BUB3 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_004725.3
Tamanho de cDNA:987bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens POT1 protection of telomeres 1 homolog with C terminal Myc tag.
Sinónimo de gene:hPot1, DKFZp586D211, POT1
Espécie:Human
Vetor:pCMV3-C-Myc
Plasmid:pCMV3-BUB3-Myc
Local de restrição:HindIII + XbaI (6kb + 1.03kb)
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with BUB3 qPCR primers for gene expression analysis, HP101180 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Humano BUB3 Gene Plasmid Map
Human BUB3 ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Humano BUB3 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc Etiqueta on other vectors
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