Bikunin qPCR Primer Pairs, Human

Cat: HP101760

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Bikunin qPCR Primer Pairs, Human General Information

Target Details

Species:
Human

Product Details

Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.

Application & Quality

Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.

Storage & Shipping

Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages

Unique Primer Design

To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.

Strict Validation Process

Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.

Uniform PCR conditions, Saving time and cost

~100% amplification curve, ensuring the accuracy of the RNA quantitative

Bikunin qPCR Primer Pairs, Human Alternative Names

A1M qPCR Primer Pairs, Human;EDC1 qPCR Primer Pairs, Human;HCP qPCR Primer Pairs, Human;HI30 qPCR Primer Pairs, Human;IATIL qPCR Primer Pairs, Human;ITI qPCR Primer Pairs, Human;ITIL qPCR Primer Pairs, Human;ITILC qPCR Primer Pairs, Human;UTI qPCR Primer Pairs, Human

Bikunin Background Information

The AMBP [A1M (alpha1-microglobulin)/bikunin precursor] gene encodes two plasma glycoproteins: A1M, an immunosuppressive lipocalin, and bikunin, a member of plasma serine proteinase inhibitor family with prototypical Kunitz-type domain. Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. AMBP mRNA is strongly transcribed in liver parenchyma, pancreas, and intestine epithelium. Sites of weaker expression are the vessels of the umbilical cord, the developing vertebral bodies, and kidney. The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. However, additional sites of protein distribution were found that do not correlate to mRNA localization: alpha(1)-microglobulin was found in myocytes and bikunin in cardiac muscle, nervous system microvasculature, and connective tissue
Full Name
alpha-1-microglobulin/bikunin precursor

Bikunin qPCR Primer Pairs, Rat, Related Products

Product Name Catalog#(PDF)
Bikunin cDNA ORF Clone in Cloning Vector, Rat RG81299-U
Bikunin cDNA ORF Clone, Rat, untagged RG81299-UT
Bikunin Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81299-ACGLN
Bikunin cDNA ORF Clone, Rat, C-GFPSpark® tag RG81299-ACG
Bikunin cDNA ORF Clone, Rat, C-OFPSpark® tag RG81299-ACR
Bikunin cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG81299-CF
Bikunin cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG81299-NF
Bikunin cDNA ORF Clone, Rat, C-His tag RG81299-CH
Bikunin cDNA ORF Clone, Rat, N-His tag RG81299-NH
Bikunin cDNA ORF Clone, Rat, C-HA tag RG81299-CY
Bikunin cDNA ORF Clone, Rat, N-HA tag RG81299-NY
Bikunin cDNA ORF Clone, Rat, C-Myc tag RG81299-CM
Bikunin cDNA ORF Clone, Rat, N-Myc tag RG81299-NM

Bikunin qPCR Primer Pairs, Mouse, Related Products

Product Name Catalog#(PDF)
Bikunin cDNA ORF Clone in Cloning Vector, Mouse MG53534-G
Bikunin cDNA ORF Clone, Mouse, untagged MG53534-UT
Bikunin cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53534-ACG
Bikunin Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53534-ACGLN
Bikunin cDNA ORF Clone, Mouse, C-OFPSpark® tag MG53534-ACR
Bikunin cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG53534-CF
Bikunin cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG53534-NF
Bikunin cDNA ORF Clone, Mouse, C-His tag MG53534-CH
Bikunin cDNA ORF Clone, Mouse, N-His tag MG53534-NH
Bikunin cDNA ORF Clone, Mouse, C-HA tag MG53534-CY
Bikunin cDNA ORF Clone, Mouse, N-HA tag MG53534-NY
Bikunin cDNA ORF Clone, Mouse, C-Myc tag MG53534-CM
Bikunin cDNA ORF Clone, Mouse, N-Myc tag MG53534-NM

Bikunin qPCR Primer Pairs, Human, Related Products

Product Name Catalog#(PDF)
Bikunin cDNA ORF Clone in Cloning Vector, Human HG13141-G
Bikunin cDNA ORF Clone, Human, untagged HG13141-UT
Bikunin Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13141-ACGLN
Bikunin cDNA ORF Clone, Human, C-GFPSpark® tag HG13141-ACG
Bikunin cDNA ORF Clone, Human, C-OFPSpark® tag HG13141-ACR
Bikunin cDNA ORF Clone, Human, C-DDK (Flag®) tag HG13141-CF
Bikunin cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13141-NF
Bikunin cDNA ORF Clone, Human, C-His tag HG13141-CH
Bikunin cDNA ORF Clone, Human, N-His tag HG13141-NH
Bikunin cDNA ORF Clone, Human, C-HA tag HG13141-CY
Bikunin cDNA ORF Clone, Human, N-HA tag HG13141-NY
Bikunin cDNA ORF Clone, Human, C-Myc tag HG13141-CM
Bikunin cDNA ORF Clone, Human, N-Myc tag HG13141-NM
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