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Ratazana GZMC/granzyme C clonagem de ADN ou de clonagem do gene (vector de expressão)

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Ratazana GZMC Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_134332.2
Tamanho de cDNA:747bp
Descrição de cDNA:Full length Clone DNA of Rattus norvegicus granzyme C.
Sinónimo de gene:Rnpk4, Rnpk-4
Espécie:Rat
Vetor:pUC19 Vector
Plasmid:cpUC19-ratGzmc
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 210A>G, 420A>G, 441C>T and 474C>A not causing the amino acid variation; 18A>G (I6M) and 31C>T (L11F) .
Sequencing primers:M13-47 and RV-M
( We provide with GZMC qPCR primers for gene expression analysis, RP300690 )
Promoter:
Application:
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Ratazana GZMC Gene Plasmid Map
Rat GZMC Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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