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Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de expressão)

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Cynomolgus GAPDH Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:XM_005569912.1
Tamanho de cDNA:1008bp
Descrição de cDNA:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) glyceraldehyde-3-phosphate dehydrogenase.
Sinónimo de gene:GAPDH
Espécie:Cynomolgus
Vetor:pGEM-T Vector
Plasmid:pGEM-cynoGAPDH
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 282T/C, 300T/C, 912C/T not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaCG90448-ACG$225
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaCG90448-ACR$225
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaCG90448-ANG$225
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaCG90448-ANR$225
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaCG90448-CF$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaCG90448-CH$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaCG90448-CM$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaCG90448-CY$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de expressão)CG90448-G$75
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaCG90448-NF$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaCG90448-NH$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaCG90448-NM$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaCG90448-NY$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem)CG90448-UT$195
 Saiba mais sobre vectores de expressão
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Fundo

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Referências
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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