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Ratazana GATE-16 / GABARAPL2 clonagem de ADN ou de clonagem do gene (vector de expressão)

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Rat GABARAPL2 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_022706.2
Tamanho de cDNA:354bp
Descrição de cDNA:Full length Clone DNA of Rattus norvegicus GABA(A) receptor-associated protein like 2.
Sinónimo de gene:Gef2
Espécie:Rat
Vetor:pUC19 Vector
Plasmid:cpUC19-ratGabarapl2
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Rat GABARAPL2 Gene Plasmid Map
Rat GABARAPL2 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Ratazana GATE-16 / GABARAPL2 clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
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Fundo

GATE-16, also known as ATG8, belongs to the MAP1 LC3 family. It is expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. GATE-16 is expressed at very low levels in lung, thymus and small intestine. GATE-16 is involved in intra-Golgi traffic. It modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

Referências
  • Ewing Rob M, et al. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol Syst Biol. 3(1):89.
  • Okazaki N, et al. (2000) Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation. Brain Res Mol Brain Res. 85(1-2):1-12.
  • Xin Y, et al. (2001) Cloning, expression patterns, and chromosome localization of three human and two mouse homologues of GABA(A) receptor-associated protein. Genomics. 74 (3):408-13.
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    Catálogo: RG80724-U
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