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Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta

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Ferret STX8 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:XM_004760175.1
Tamanho de cDNA:711bp
Descrição de cDNA:Full length Clone DNA of Mustela putorius furo (sub-species: furo) syntaxin 8 with N terminal Flag tag.
Sinónimo de gene:STX8
Espécie:Ferret
Vetor:pCMV3-N-FLAG
Plasmid:
Local de restrição:
Sequência de etiqueta:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag Etiqueta on other vectors
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaFG60077-ACG$225
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaFG60077-ACR$225
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaFG60077-ANG$225
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaFG60077-ANR$225
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaFG60077-CF$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaFG60077-CH$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaFG60077-CM$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaFG60077-CY$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de expressão)FG60077-G$75
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaFG60077-NF$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaFG60077-NH$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaFG60077-NM$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaFG60077-NY$195
Furão STX8 / Syntaxin 8 clonagem de ADN ou de clonagem do gene (vector de clonagem)FG60077-UT$195
 Saiba mais sobre vectores de expressão
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Fundo

STX8, also known as syntaxin 8, directly interacts with HECTd3. STX8 forms the SNARE complex with syntaxin 7, vti1b and endobrevin. STX8 belongs to the syntaxin family. Members of this family are key molecules implicated in diverse vesicle docking and membrane fusion events. STX8 physically interacts with cystic fibrosis transmembrane conductance regulator (CFTR): recombinant syntaxin 8 binds CFTR in vitro and both proteins co-immunoprecipitate in HT29 cells. Syntaxin 8 regulates CFTR-mediated currents in chinese hamster ovary (CHO) cells stably expressing CFTR and syntaxin 8. STX8 contributes to the regulation of CFTR trafficking and chloride channel activity by the SNARE machinery.

Referências
  • Steegmaier M. et al., 1999, J Biol Chem. 273 (51): 34171-9.
  • Thoreau V. et al., 1999, Biochem Biophys Res Commun. 257 (2): 577-83.
  • Zhang L. et al., 2009, Cell Mol Neurobiol. 29 (1): 115-21.
  • Bilan F. et al., 2004, J Cell Sci. 117 (10): 1923-35.
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