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Humano DGKG clonagem de ADN ou de clonagem do gene (vector de expressão)

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Human DGKG Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:BC112363
Tamanho de cDNA:2259bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens diacylglycerol kinase, gamma 90kDa.
Sinónimo de gene:DAGK3, DGK-GAMMA
Espécie:Human
Vetor:pGEM-T Vector
Plasmid:pGEM-DGKG
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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