Encomenda rápida

Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA Etiqueta

    Folha de dadosAnálisesProdutos relacionadosProtocolos
    Rhesus HSP90AA1 Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:NM_001195667.1
    Tamanho de cDNA:2202bp
    Descrição de cDNA:Full length Clone DNA of Macaca mulatta (Rhesus monkey) heat shock protein 90kDa alpha (cytosolic), class A member 1 with C terminal HA tag.
    Sinónimo de gene:HSP90AA1
    Espécie:Rhesus
    Vetor:pCMV3-C-HA
    Plasmid:
    Local de restrição:
    Sequência de etiqueta:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    Descrição da sequência:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA Etiqueta on other vectors
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaCG90401-ACG$245
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaCG90401-ACR$245
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaCG90401-ANG$245
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaCG90401-ANR$245
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaCG90401-CF$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaCG90401-CH$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaCG90401-CM$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaCG90401-CY$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de expressão)CG90401-G$75
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaCG90401-NF$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaCG90401-NH$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaCG90401-NM$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaCG90401-NY$215
    Rhesus HSP90/HSP90AA1 clonagem de ADN ou de clonagem do gene (vector de clonagem)CG90401-UT$215
     Saiba mais sobre vectores de expressão
    Product nameProduct name
    Fundo

    Heat shock protein 90 (90 kDa heat-shock protein, HSP90) is a molecular chaperone involved in the trafficking of proteins in the cell. It is a remarkably versatile protein involved in the stress response and in normal homoeostatic control mechanisms. HSP90 interacts with 'client proteins', including protein kinases, transcription factors and others, and either facilitates their stabilization and activation or directs them for proteasomal degradation. By this means, HSP90 displays a multifaceted ability to influence signal transduction, chromatin remodelling and epigenetic regulation, development and morphological evolution. HSP90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis at the N-terminus. Disruption of HSP90 leads to client protein degradation and often cell death. Under stressful conditions, HSP90 stabilizes its client proteins and provides protection to the cell against cellular stressors such as in cancer cells. Especially, several oncoproteins act as HSP90 client proteins and tumor cells require higher HSP90 activity than normal cells to maintain their malignancy. For this reason, Hsp90 has emerged as a promising target for anti-cancer drug development.

    Referências
  • Pearl LH, et al. (2008) The Hsp90 molecular chaperone: an open and shut case for treatment. Biochem J. 410(3): 439-53.
  • Hahn JS. (2009) The Hsp90 chaperone machinery: from structure to drug development. BMB Rep. 42(10): 623-30.
  • Holzbeierlein JM, et al. (2010) Hsp90: a drug target? Curr Oncol Rep. 12(2): 95-101.
  • Trepel J, et al. (2010) Targeting the dynamic HSP90 complex in cancer. Nat Rev Cancer. 10(8): 537-49.
  • Size / Price
    Catálogo: CG90401-CY
    Preço de catálogo: 
    Preço:      (You Save: )
    Tamanho:
    Quantidade:+-
    Disponibilidade
    Acrescentar a carrinhoBulk Discount Requiry

    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Tenha em atenção o seguinte: Todos os produtos destinam-se "A FINS EXCLUSIVOS DE INVESTIGAÇÃO E NÃO DEVEM SER UTILIZADOS PARA EFEITOS DE DIAGNÓSTICO OU TERAPÊUTICOS"