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Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc Etiqueta

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Cynomolgus GAPDH Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:XM_005569912.1
Tamanho de cDNA:1008bp
Descrição de cDNA:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) glyceraldehyde-3-phosphate dehydrogenase with C terminal Myc tag.
Sinónimo de gene:GAPDH
Espécie:Cynomolgus
Vetor:pCMV3-C-Myc
Plasmid:
Local de restrição:
Sequência de etiqueta:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descrição da sequência:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc Etiqueta on other vectors
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaCG90448-ACG$225
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Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaCG90448-ANR$225
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaCG90448-CF$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaCG90448-CH$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaCG90448-CM$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaCG90448-CY$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de expressão)CG90448-G$75
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaCG90448-NF$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaCG90448-NH$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaCG90448-NM$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaCG90448-NY$195
Cynomolgus GAPDH clonagem de ADN ou de clonagem do gene (vector de clonagem)CG90448-UT$195
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Fundo

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Referências
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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