|Folha de dados||Análises||Produtos relacionados||Protocolos|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus CSNK2A1 ORF mammalian expression plasmid, C-GFPSpark tag||CG90444-ACG|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90444-ACR|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, N-GFPSpark tag||CG90444-ANG|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||CG90444-ANR|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, C-Flag tag||CG90444-CF|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, C-His tag||CG90444-CH|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, C-Myc tag||CG90444-CM|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, C-HA tag||CG90444-CY|
|Rhesus CSNK2A1 Gene cDNA clone plasmid||CG90444-G|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, N-Flag tag||CG90444-NF|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, N-His tag||CG90444-NH|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, N-Myc tag||CG90444-NM|
|Rhesus CSNK2A1 ORF mammalian expression plasmid, N-HA tag||CG90444-NY|
|Rhesus CSNK2A1 natural ORF mammalian expression plasmid||CG90444-UT|
|Saiba mais sobre vectores de expressão|
Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.