|Folha de dados||Análises||Produtos relacionados||Protocolos|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine IL12B ORF mammalian expression plasmid, C-GFPSpark tag||DG70011-ACG|
|Canine IL12B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70011-ACR|
|Canine IL12B ORF mammalian expression plasmid, C-Flag tag||DG70011-CF|
|Canine IL12B ORF mammalian expression plasmid, C-His tag||DG70011-CH|
|Canine IL12B ORF mammalian expression plasmid, C-Myc tag||DG70011-CM|
|Canine IL12B ORF mammalian expression plasmid, C-HA tag||DG70011-CY|
|Canine IL12B ORF mammalian expression plasmid, N-Flag tag||DG70011-NF|
|Canine IL12B ORF mammalian expression plasmid, N-His tag||DG70011-NH|
|Canine IL12B ORF mammalian expression plasmid, N-Myc tag||DG70011-NM|
|Canine IL12B ORF mammalian expression plasmid, N-HA tag||DG70011-NY|
|Canine IL12B natural ORF mammalian expression plasmid||DG70011-UT|
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Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity.