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Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His Etiqueta

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Human CLU Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_001831.2
Tamanho de cDNA:1506bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens clusterin with His tag.
Sinónimo de gene:CLI, AAG4, APOJ, KUB1, SGP2, SGP-2, SP-40, TRPM2, TRPM-2, MGC24903, CLU
Espécie:Human
Vetor:pCMV/hygro-His
Plasmid:pCMV-CLU-10his
Local de restrição:KpnI + XhoI (5.5kb + 1.54kb)
Sequência de etiqueta:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Descrição da sequência:Identical with the Gene Bank Ref.ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Human CLU Gene Plasmid Map
Human CLU Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
pCMV/hygro-HIs Vector Information
 
Vector Name pCMV/hygro-His
Vector Size 5687bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG11297-ACG$245
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG11297-ACR$245
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG11297-CF$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG11297-CH$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG11297-CM$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG11297-CY$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de expressão)HG11297-M$75
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG11297-NF$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG11297-NH$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG11297-NM$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG11297-NY$215
Humano Clusterin/Apolipoprotein J/Apo-J clonagem de ADN ou de clonagem do gene (vector de clonagem)HG11297-UT$215
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Fundo

Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

Referências
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.
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