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Humano BTNL3 clonagem de ADN ou de clonagem do gene (vector de expressão)

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    Humano BTNL3 Informações sobre o produto de clone de cDNA
    Gene_bank_ref_id:BC100801
    Tamanho de cDNA:1401bp
    Descrição de cDNA:Full length Clone DNA of Homo sapiens butyrophilin-like 3.
    Sinónimo de gene:BTNLR
    Espécie:Human
    Vetor:pGEM-T Vector
    Plasmid:pGEM-BTNL3
    Local de restrição:
    Sequência de etiqueta:
    Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with BTNL3 qPCR primers for gene expression analysis, HP104360 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    Fundo

    Butyrophilins (Btns) and butyrophilin-like (Btnl) molecules are emerging as novel regulators of immune responses in mice and humans.The human genome contains four identified BTNL genes, with the somewhat confusing designations of 2, 3, 8 and 9. BTNL2, the best charac- terised family member, is clustered with the BTN genes on chromosome 6, whereas the much less explored family members BTNL3, BTNL8 and BTNL9 are localised on chromosome 5.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    Referências
  • Abeler-Dörner L, Swamy M, Williams G, et al. Butyrophilins: an emerging family of immune regulators[J]. Trends in immunology, 2012, 33(1): 34-41.
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    Catálogo: HG15769-G
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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