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Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de expressão)

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Rat ARG1 Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_017134.3
Tamanho de cDNA:972bp
Descrição de cDNA:Full length Clone DNA of Rattus norvegicus arginase 1.
Sinónimo de gene:Arg1
Espécie:Rat
Vetor:pUC19 Vector
Plasmid:cpUC19-ratArg1
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 146A>G(D49G) and 836A>G(N279S).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
Rat ARG1 Gene Plasmid Map
Rat ARG1 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaRG81309-ACG$225
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaRG81309-ACR$225
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-GFPSpark EtiquetaRG81309-ANG$225
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-OFPSpark EtiquetaRG81309-ANR$225
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaRG81309-CF$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaRG81309-CH$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaRG81309-CM$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaRG81309-CY$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaRG81309-NF$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaRG81309-NH$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaRG81309-NM$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaRG81309-NY$195
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de expressão)RG81309-U$75
Ratazana ARG1/Arginase 1 clonagem de ADN ou de clonagem do gene (vector de clonagem)RG81309-UT$195
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Fundo

Arginase is the focal enzyme of the urea cycle hydrolysing L-arginine to urea and L-ornithine. Emerging studies have identified arginase in the vasculature and have implicated this enzyme in the regulation of nitric oxide (NO) synthesis and the development of vascular disease. Arginase also redirects the metabolism of L-arginine to L-ornithine and the formation of polyamines and L-proline, which are essential for smooth muscle cell growth and collagen synthesis. Arginase is encoded by two recently discovered genes (Arginase I and Arginase II). In most mammals, Arginase 1 (ARG1) also known as Arginase, liver, which functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain.

Referências
  • Durante W, et al. (2007) Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 34(9): 906-11.
  • Waddington SN. (2002) Arginase in glomerulonephritis. Kidney Int. 61(3): 876-81.
  • Morris SM. (2002). Regulation of enzymes of the urea cycle and arginine metabolism. Annual review of nutrition. 22 (1): 87-105.
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