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Humano ACSM2A clonagem de ADN ou de clonagem do gene (vector de expressão)

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Humano ACSM2A Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:BC125176
Tamanho de cDNA:1734bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens acyl-CoA synthetase medium-chain family member 2A.
Sinónimo de gene:ACSM2, A-923A4.1
Espécie:Human
Vetor:pGEM-T Vector
Plasmid:pGEM-ACSM2A
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
( We provide with ACSM2A qPCR primers for gene expression analysis, HP104485 )
Promoter:
Application:
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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