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Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de expressão)

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Human ACHE Informações sobre o produto de clone de cDNA
Gene_bank_ref_id:NM_000665.3
Tamanho de cDNA:1845bp
Descrição de cDNA:Full length Clone DNA of Homo sapiens acetylcholinesterase.
Sinónimo de gene:YT, ACEE, ARACHE, N-ACHE
Espécie:Human
Vetor:pGEM-T Vector
Plasmid:pGEM-ACHE-E4-E6
Local de restrição:
Sequência de etiqueta:
Descrição da sequência:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Armazenamento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de expressão) on other vectors
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-GFPSpark EtiquetaHG15847-ACG$245
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-OFPSpark EtiquetaHG15847-ACR$245
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Flag EtiquetaHG15847-CF$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-His EtiquetaHG15847-CH$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-Myc EtiquetaHG15847-CM$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), C-HA EtiquetaHG15847-CY$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Flag EtiquetaHG15847-NF$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-His EtiquetaHG15847-NH$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-Myc EtiquetaHG15847-NM$215
Humano Acetylcholinesterase transcript variant E4-E6 clonagem de ADN ou de clonagem do gene (vector de clonagem), N-HA EtiquetaHG15847-NY$215
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Fundo

Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2% of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.

Referências
  • Zhao Y, et al. (2011) Acetylcholinesterase, a key prognostic predictor for hepatocellular carcinoma, suppresses cell growth and induces chemosensitization. Hepatology. 53(2): 493-503.
  • Roepcke CB, et al. (2010) Analysis of phosphorothionate pesticides using a chloroperoxidase pretreatment and acetylcholinesterase biosensor detection. J Agric Food Chem. 58(15): 8748-56.
  • Zaheer-ul-Haq, et al. (2010) Benchmarking docking and scoring protocol for the identification of potential acetylcholinesterase inhibitors. J Mol Graph Model. 28(8): 870-82.
  • Pegan K, et al. (2010) Acetylcholinesterase is involved in apoptosis in the precursors of human muscle regeneration. Chem Biol Interact. 187(1-3): 96-100.
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